cy5 5 dye  (MedChemExpress)

Journal: Materials Today Bio

Article Title: Self-assembled nanoparticles from Xiexin Decoction attenuate ulcerative colitis by targeting VDAC1-Mediated NLRP3 inflammasome activation

doi: 10.1016/j.mtbio.2026.103078

Figure Lengend Snippet: Gastrointestinal stability and biodistribution of RBNPs. (A – C) Hydrodynamic diameter (A), PDI (B), and zeta potential (C) of RBNPs incubated in PBS, SGF, or SIF. (D) In vitro cumulative release profiles of BBR from RBNPs in SGF and SIF over 48 h. (E – F) Representative in vivo fluorescence images (E) and quantification of radiant efficiency (F) in mice following oral administration of Cy5.5@RBNPs or free Cy5.5. (G) Ex vivo fluorescence images of major organs (H, heart; Lu, lung; Li, liver; S, spleen; K, kidney) and colons excised at indicated time points. (H) Quantification of radiant efficiency in major organs and colons. Data are presented as mean ± SD ( n = 3). Statistical significance was assessed by one-way ANOVA with Tukey's post hoc test, ∗∗∗∗ p < 0.0001; ns, not significant.

Article Snippet: MedChemExpress supplied the Cy5.5 dye.

Techniques: Zeta Potential Analyzer, Incubation, In Vitro, In Vivo, Fluorescence, Ex Vivo

Journal: Materials Today Bio

Article Title: Self-assembled nanoparticles from Xiexin Decoction attenuate ulcerative colitis by targeting VDAC1-Mediated NLRP3 inflammasome activation

doi: 10.1016/j.mtbio.2026.103078

Figure Lengend Snippet: In vitro anti-inflammatory effects of XXD-based self-assembled nanoparticles. (A) Viability of RAW264.7 cells after 24-h treatment with XXD, XDNPs, or RBNPs, assessed using the CCK-8 assay. (B – C) Flow cytometry analysis of cellular uptake of Cy5.5-labeled RBNPs (Cy5.5@RBNPs) versus free Cy5.5. (D – E) Flow cytometry assessment of macrophage polarization (M1/M2) in RAW264.7 cells following treatment with the indicated formulations. (F – H) ELISA quantification of pro-inflammatory cytokines IL-1β (F), IL-6 (G), and TNF-α (H) in culture supernatants. (I) Nitric oxide (NO) production in the supernatant. (J) Confocal laser scanning microscopy (CLSM) images. (K – L) Flow cytometry analysis of intracellular reactive oxygen species (ROS). (M) Intracellular hydrogen peroxide (H 2 O 2 ) levels in LPS-stimulated RAW264.7 cells after treatment with the formulations. Data are presented as mean ± SD ( n = 3). Statistical significance was calculated using one-way ANOVA with Tukey's post hoc test. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001 versus the Model group; #### p < 0.0001 versus the Ctrl group.

Article Snippet: MedChemExpress supplied the Cy5.5 dye.

Techniques: In Vitro, CCK-8 Assay, Flow Cytometry, Labeling, Enzyme-linked Immunosorbent Assay, Confocal Laser Scanning Microscopy

Journal: Journal of Nanobiotechnology

Article Title: Platinum-doped emodin carbon dots mitigate sepsis-induced lung injury by targeting the gut-lung axis

doi: 10.1186/s12951-025-03972-0

Figure Lengend Snippet: Pt-ECDs Mitigate Systemic Pathological Manifestations in Septic Mice. ( A ) Schematic illustration of CLP-induced sepsis establishment and Pt-ECDs therapeutic protocol. ( B ) Representative intestinal fluorescence imaging in mice following oral gavage of Cy5.5-labeled Pt-ECDs at 4, 8, 12, 24, and 48 h post-CLP ( n = 3 biological replicates per group). ( C-F ) Physiological indicators at 24 h post-CLP: ( C ) Survival rate, ( D ) Body weight variation, ( E ) Clinical score, and ( F ) Body temperature in the control group, sepsis group (24 h post-CLP), and Pt-ECDs treatment group (5 µg/kg) ( n = 10 biological replicates per group). ( G-R ) Expression profiles of serum biochemical markers among three groups including ( G ) WBC, ( H ) Neut, ( I ) ALT, ( J ) AST, ( K ) BUN, ( L ) Cr, ( M ) TNF-α, and ( N ) IL-1β, ( O ) IL-6, ( P ) IL-10, ( Q ), IFN-γ, ( R ) IFN-β ( n = 6 biological replicates per group). ( S ) Lung wet/dry weight ratio among three groups ( n = 6 biological replicates per group). ( T ) Lung injury scores among three groups ( n = 6 biological replicates per group). ( U ) Quantitative analysis of LDH activity among three groups ( n = 6 biological replicates per group). ( V ) Representative H&E-stained lung tissue sections from the three groups (scale bar: 100 μm; n = 6 biological replicates per group). ( W-X ) Representative images of intestinal reactive oxygen species (ROS) fluorescence staining ( W ) and quantitative analysis ( X ) of ROS production capacity ( n = 3 biological replicates per group). ( Y ) Representative western blot analysis of intestinal tight junction proteins (ZO-1 and CLAUDIN-1) ( n = 3 biological replicates per group). (Z-AA) Representative immunofluorescence (IF) images of ( Z ) CLDN1 and (AA) ZO-1 proteins (scale bar: 100 μm; n = 3 biological replicates per group). Data were expressed as mean ± SD (Significance was calculated by one-way ANOVA with Sidakʼs multiple-comparisons test, * P-value < 0.05; ** P-value < 0.01; *** P-value < 0.001;**** P-value < 0.0001 )

Article Snippet: Probe preparation involved vigorous mixing of Pt-ECDs or PBS with near-infrared dye Cy5.5 (MedChemExpress HY-D0924, USA) followed by overnight incubation at 4 °C.

Techniques: Fluorescence, Imaging, Labeling, Control, Expressing, Activity Assay, Staining, Western Blot, Immunofluorescence